I was able to cycle a 29 gallon in 3 weeks seeding it with 2 nylon stockings full of gravel from my established 55 gal tank hanging on the sides... along with putting gravel from the established tank and a few stones from the established tanks in the 29 filter. So three weeks and 2 days later ... thats 23 days
the tank is cycled. ammonia = 0 nitrite = zero and nitrates = 20 by the way its an aqua clear filter 30 gal
So i want to start to add fish slowly. Right now there are 6 zebra danios in there.
The question is what do I do with the substrate i have hanging in the tank in the stockings and the subsrate i put in the filter. if i take them out will there be a mini cycle? They is gravel in the nylon and the new tank subtrate is sand so i am not going to mix them.
Also, is it safe to put the subtrate i used to cycle the 29 gal back to the 55 gal or should i just toss it ?

So i want to start to add fish slowly. Right now there are 6 zebra danios in there.
The question is what do I do with the substrate i have hanging in the tank in the stockings and the subsrate i put in the filter. if i take them out will there be a mini cycle? They is gravel in the nylon and the new tank subtrate is sand so i am not going to mix them.
Also, is it safe to put the subtrate i used to cycle the 29 gal back to the 55 gal or should i just toss it ?